Detection and Characterization of the Toxic Shock Syndrome (TSST-1) Toxin Gene Staphylococcus aureus in Isolates of Patients with Urinary Tract Infections

Abstract

Staphylococcus aureus is a bacteria that causes urinary tract infections. One of the toxins produced by S. aureus is toxic shock syndrome toxin-1 (TSST-1), which can cause multi-organ abnormalities. This disorder is characterized by the onset of fever, hypotension, disorders of the digestive organs, endothelial cells and vascular muscles. The aim of this study was to detect the presence of the TSST-1 gene encoding S. aureus isolates in isolates of patients with urinary tract infections. This research began with the conventional re-identification stage of S. aureus, followed by molecular-based identification using the PCR method. A total of 25 S. aureus isolates were cultured aerobically, followed by gram staining, catalase, coagulase, MSA, MR, Indole and VP tests. Molecular-based identification of S. aureus was carried out by amplifying the 16S rRNA gene, followed by amplifying the gene encoding TSST-1 as the target gene. PCR product of the gene encoding TSST-1. The results showed that conventional re-identification of S. aureus resulted in 100% visibility of the position of S. aureus. Molecular-based identification by amplifying the 16S rRNA gene gave good results, with the discovery of a DNA fragment measuring 745 bp, according to the target gene of 2 isolates (8%). As conclusion, 8% of S. aureus bacteria were positive for the tsst-1 gene.

Keywords: Staphylococcus aureus; toxic shock syndrome toxin-1 (TSST-1); urinary tract infections